Ikhithi yokutholwa kwe-PCR ye-SARS-CoV-2 Constant Temperature (Ukusetshenziswa kwasekhaya)

Incazelo emfushane:

Isingeniso somkhiqizo:

Isetshenziselwa ukuhlola isampula ukuze ihlolwe indawo ekhethekile yenoveli coronavirus nucleic acids (ORF1ab,Ngene).

Izici zomkhiqizo:

•Kulula :Kulula ukusebenzisa, ukufunda, nokuqonda, akukho ukuqeqeshwa okuyinkimbinkimbi okudingekayo.
•I-Isothermal :Londoloza izindleko zensimbi.
•Ukucaciswa okuphezulu:Dukunemba kwe-tection kuze kufike ku-98%.
•Kushesha :Ukutholwa kungaqedwa phakathi nemizuzu engu-15.
•Izokuthutha nesitoreji esikahle :izinga lokushisa lokuhamba nokugcinwa kwegumbi, alikho iketango elibandayo.

Imininingwane yomkhiqizo:

1 ukuhlolwa/ibhokisi, izivivinyo eziyi-16/ibhokisi

①swab②ishubhu yokulondoloza iswab③Amplification reaction tube④ibhavu lensimbi


  • Igama lomkhiqizo:Ikhithi yokutholwa kwe-PCR ye-SARS-CoV-2 Constant Temperature (Ukusetshenziswa kwasekhaya)
  • Uhlobo:Izinga Lokushisa Ezihlala njalo PCR
  • Ukucaciswa kokupakisha:Isivivinyo esi-1/ibhokisi, izivivinyo eziyi-16/ibhokisi
  • Imininingwane Yomkhiqizo

    Omaka bomkhiqizo

    Umgomo wokuhlola:
    Le kit ithola i-RNA ye-SARS-CoV-2 isebenzisa indlela yokukhulisa i-isothermal.Ukuloba okuhlanekezelwe kanye nokwandiswa kwe-RNA kwenziwa kushubhu efanayo.Ukulandelana kwe-nucleic acid kwe-SARS-CoV-2 kukhonjwa ngokuqondile ngama-primer ayisithupha, futhi noma yikuphi ukungafani kwe-primer noma okungabhanqiwe ngeke kuqedele ukukhulisa.Wonke ama-reagents nama-enzyme adingekayo ekuphenduleni alayishwa ngaphambilini.Inqubo elula iyadingeka futhi umphumela ungatholwa ngokubheka ubukhona noma cha be-fluorescence.

    Ukulungiselela:

    Vula isikhwama se-Aluminium foil bese ukhipha amashubhu okusabela.Qaphela, ishubhu yokusabela kufanele isetshenziswe kungakapheli amahora ama-2 uma isikhwama sayo se-foil sesivuliwe.

    Xhuma amandla.Ithuluzi liqala ukushisa (Inkomba yokushisisa iba bomvu futhi ikhanyise).Ngemuva kwenqubo yokushisa, inkomba yokushisa iphenduka ibe luhlaza ngebhiphu.

    Iqoqo lesampula:

    Tshekisa ikhanda lesiguli emuva cishe ngo-70 °, Vumela ikhanda lesiguli likhululeke ngokwemvelo , futhi kancane uphendukise i-swab odongeni lwe-ostril ekhaleni lesiguli kuya kulwanga lwamakhala, bese uyisusa kancane ngenkathi usula.

    Isivivinyo:
    ①Dabula ifilimu ye-aluminium foil seal yeshubhu le-Swab Preservation, bese ufaka i-swab eshubhuni yokulondoloza i-swab.ngenkathi ucindezela i-tube, gxuma i-swab.
    ②Susa i-swab ngenkathi ucindezela izinhlangothi zeshubhu ukuze ukhiphe uketshezi ku-swab.
    ③Khama i-micropipette bese uyifaka oketshezini.Dedela i-micropi-pette ukuze udonse uketshezi kuze kube yilapho uketshezi lugelezela ku-capsule yokuqala.Ungavumeli uketshezi lugcwalise i-capsule yokuqala.
    ④Engeza uketshezi lwesampula eshubhuni yokusabela, Vala ikepisi, xuba ngobumnene ingxube ize incibilike ngokuphelele.
    ⑤Vula ikhava yokugeza okomile.Faka amashubhu okusabela anekepisi kubhavu owomile.Cindezela inkinobho yesikhathi.Inkomba yokushisisa eluhlaza iqala ukukhanya.Ngemuva kwemizuzu engu-15, ukusabela kuyaqedwa.Isibonisi sokushisisa esiluhlaza siyayeka ukucwayiza ngamabhiphu amathathu.
    ⑥Cindezela inkinobho yokushintsha yomthombo wokukhanya bese ubheka imiphumela yokuhlola ngembobo yokubuka ephambi kokugeza okomile ukuze wahlulele imiphumela.
    Ukuhunyushwa kwemiphumela yokuhlolwa:

    Umphumela omuhle: uma ishubhu lokusabela linesasasa elisobala le-fluorescence eliluhlaza, umphumela uba positive.Isiguli kusolwa ukuthi singenwe i-Sars-Cov-2.Ngokushesha thintana nodokotela noma umnyango wezempilo wendawo futhi ulandele imihlahlandlela yendawo.
    Umphumela ongemuhle: uma ishubhu lokusabela lingenayo i-excitation ye-fluorescence esobala, umphumela uba negative.Qhubeka nokunamathela kuyo yonke imithetho esebenzayo mayelana nokuxhumana nabanye kanye nezinyathelo zokuzivikela.Kungase futhi kube khona ukutheleleka lapho kuhlolwa ukuthi awunayo.
    Umphumela ongavumelekile: uma isikhathi sokufukamela singaphezu kwamaminithi angu-20, ukukhuliswa okungaqondile kungase kwenzeke, okuholela kuphozithivu engelona iqiniso.Kuyobe kungavumelekile kungakhathaliseki ukuthi kukhona i-fluorescence esobala, futhi ukuhlolwa kuzophinda kwenziwe.







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